Ultraviolet-crosslinking reveals specific affinity of eukaryotic initiation factors for Semliki Forest virus mRNA
Publication date
1984
Authors
Voorma, H.O.
Setyono, B.
Steeg, H. van
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Document Type
Article
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Abstract
Eukaryotic initiation factors (elF) associate readily with ³²P-labeled Semliki Forest virus (SFV) mRNA in
vitro, forming complexes which can be crosslinked by 254 nm ultraviolet irradiation. After ribonuclease
digestion, the initiation factors were released and analysed by gel electrophoresis. Autoradiography revealed
proteins by virtue of crosslinked ³²P-labeled mRNA fragments, elF-4A, -4B and -4C as well as three subunits
of elF-3 could be crosslinked with SFV mRNA. None of these proteins bound to ribosomal RNAs.
Keywords
Protein synthesis; Initiation factor; Ultraviolet crosslinking; mRNA; (Semliki Forest virus), protein synthesis, initiation factor, ultraviolet crosslinking, mRNA, (Semliki Forest virus)