Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins
Publication date
2021
Authors
Luo, Yanzhang
Xiang, Shengqi
Lucini Paioni, Alessandra
Adler, Agnes
Hooikaas, Peter Jan
Jijumon, A S
Janke, Carsten
Akhmanova, A.S.
Baldus, M.
Editors
Owens, Raymond J.
Advisors
Supervisors
Document Type
Part of book
Metadata
Show full item recordCollections
License
taverne
Abstract
In this chapter, we describe the preparatory and spectroscopic procedures for conducting solid-state NMR experiments on microtubules (MTs) obtained from human cells and their complexes with microtubule-associated proteins (MAPs). Next to labeling and functional assembly of MTs and MT-MAP complexes, we discuss solid-state NMR approaches, including fast MAS and hyperpolarization methods that can be used to examine these systems. Such studies can provide novel insight into the dynamic properties of MTs and MT-MAP complexes.
Keywords
Microtubule-associated proteins, Microtubules, Protein dynamics, Protein interactions, Solid-state NMR spectroscopy, Taverne, Molecular Biology, Genetics
Citation
Luo, Y, Xiang, S, Paioni, A L, Adler, A, Hooikaas, P J, Jijumon, A S, Janke, C, Akhmanova, A & Baldus, M 2021, Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins. in R J Owens (ed.), Structural Proteomics : High-Throughput Methods. 3 edn, Methods in Molecular Biology, vol. 2305, Humana Press, pp. 193-201. https://doi.org/10.1007/978-1-0716-1406-8_10