Super-Resolution Imaging of Intracellular Lipid Nanocarriers to Study Drug Delivery in Photodynamic Therapy
Publication date
2022-05-04
Authors
Scutigliani, Enzo M.
Kochan, Jakub A.
Desclos, Emilie C.B.
Jonker, Art
Heger, Michal
Krawczyk, Przemek M.
Editors
Broekgaarden, Mans
Zhang, Hong
Korbelik, Mladen
Hamblin, Michael R.
Heger, Michal
Advisors
Supervisors
Document Type
Part of book
Metadata
Show full item recordCollections
License
taverne
Abstract
Liposomal nanocarriers are intensively investigated as delivery vehicles for photoactivatable agents used in photodynamic therapy (PDT). The uptake, intracellular distribution, and processing of the nanocarriers are of paramount importance for the effectiveness of the therapy; visualization and analysis of these processes can, therefore, stimulate the development of improved PDT modalities. Here we describe a simple protocol, based on super-resolution imaging, that can be used for detailed quantification of concentration, distribution, and size of individual lipid nanocarriers in adherent mammalian cells.
Keywords
Photodynamic therapy, Photosensitizers, Super-resolution microscopy, Taverne, Molecular Biology, Genetics
Citation
Scutigliani, E M, Kochan, J A, Desclos, E C B, Jonker, A, Heger, M & Krawczyk, P M 2022, Super-Resolution Imaging of Intracellular Lipid Nanocarriers to Study Drug Delivery in Photodynamic Therapy. in M Broekgaarden, H Zhang, M Korbelik, M R Hamblin & M Heger (eds), Photodynamic Therapy : Methods and Protocols. 1 edn, Methods in Molecular Biology, vol. 2451, Humana Press, New York, pp. 703-709. https://doi.org/10.1007/978-1-0716-2099-1_37