Specific serology for emerging human coronaviruses by protein microarray
Publication date
2013
Authors
Reusken, C
Mou, H
Godeke, G J
van der Hoek, L
Meyer, B
Müller, M A
Haagmans, B
de Sousa, R
Schuurman, Nancy N.M.P.
Dittmer, U
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Document Type
Article
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Abstract
We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.
Keywords
Coronavirus, Coronavirus Infections, Female, Humans, Male, Protein Array Analysis, Sequence Homology, Amino Acid
Citation
Reusken, C, Mou, H, Godeke, G J, van der Hoek, L, Meyer, B, Müller, M A, Haagmans, B, de Sousa, R, Schuurman, N, Dittmer, U, Rottier, P, Osterhaus, A, Drosten, C, Bosch, B J & Koopmans, M 2013, 'Specific serology for emerging human coronaviruses by protein microarray', Eurosurveillance, vol. 18, no. 14, 20441. https://doi.org/10.2807/1560-7917.ES2013.18.14.20441