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A Robust Post-Insertion Method for the Preparation of Targeted Lipid Nanoparticles

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978-1-0716-4394-5_18.pdf (610.09 KB)

Publication date

2025-04-03

Authors

Bimbo, Jaime Fernández
Schiffelers, RaymondISNI 0000000045237985

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DOI

https://doi.org/10.1007/978-1-0716-4394-5_18

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Part of book

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Utrecht University Repository
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taverne

Abstract

Lipid nanoparticles (LNPs) have garnered significant attention as nonviral vectors for gene delivery due to their low immunogenicity and cytotoxicity. In addition, their surfaces can readily be functionalized with targeting moieties. Here we describe a simple and robust method for the preparation of targeted siRNA, mRNA, or DNA LNPs. After conjugating a targeting ligand to a PEG lipid, this binder-lipid conjugate is post-inserted into preformed LNPs. This process allows the LNPs to be easily decorated with one or various combinations of ligands.

Keywords

Binders, Gene delivery, Lipid nanoparticles (LNPs), Nanomedicine, Particle functionalization, Post-insertion, Specificity, Targeted delivery, Targeting ligand, Taverne, Molecular Biology, Genetics

Citation

Bimbo, J F & Schiffelers, R 2025, A Robust Post-Insertion Method for the Preparation of Targeted Lipid Nanoparticles. in DNA Nanotechnology. vol. 2901, Methods in Molecular Biology, vol. 2901, Humana Press, pp. 241-250. https://doi.org/10.1007/978-1-0716-4394-5_18

URI

https://dspace.library.uu.nl/handle/1874/482827