Controlled Chemoenzymatic Synthesis of Heparan Sulfate Oligosaccharides
Publication date
2018-05
Authors
Lu, Weigang
Zong, Chengli
Chopra, Pradeep
Pepi, Lauren
Xu, Yongmei
Liu, Jian
Amster, I. Jonathan
Boons, Geert-Jan
Editors
Advisors
Supervisors
Document Type
Article
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taverne
Abstract
A chemoenzymatic approach has been developed for the preparation of diverse libraries of heparan sulfate (HS) oligosaccharides. It employs chemically synthesized oligosaccharides having a chemical entity at a GlcN residue, which in unanticipated manners influences the site of modification of NST, C5-Epi/2-OST and 6-OST1/6-OST3 resulting in oligosaccharides differing in N/O-sulfation and epimerization pattern. The enzymatic transformations defined fine substrate requirements of NST, C5-Epi, 2-OST and 6-OST.
Keywords
Journal Article, Taverne
Citation
Lu, W, Zong, C, Chopra, P, Pepi, L, Xu, Y, Liu, J, Amster, I J & Boons, G-J 2018, 'Controlled Chemoenzymatic Synthesis of Heparan Sulfate Oligosaccharides', Angewandte Chemie-International Edition, vol. 57, no. 19, pp. 5340-5344. https://doi.org/10.1002/anie.201800387