Flexibility in crosstalk between H2B ubiquitination and H3 methylation in vivo (Corrigendum)
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Publication date
2014
Authors
Vlaming, Hanneke
van Welsem, Tibor
de Graaf, Erik L.
Ontoso, David
Altelaar, A.F. Maarten
San-Segundo, Pedro A
Heck, Albert
van Leeuwen, Fred
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Article
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Abstract
Histone H2B ubiquitination is a dynamic modification that promotes methylation of histone H3K79 and H3K4. This crosstalk is important for the DNA damage response and has been implicated in cancer. Here, we show that in engineered yeast strains, ubiquitins tethered to every nucleosome promote H3K79 and H3K4 methylation from a proximal as well as a more distal site, but only if in a correct orientation. This plasticity indicates that the exact location of the attachment site, the native ubiquitin-lysine linkage and ubiquitination cycles are not critical for trans-histone crosstalk in vivo. The flexibility in crosstalk also indicates that other ubiquitination events may promote H3 methylation.
Keywords
SDG 3 - Good Health and Well-being
Citation
Vlaming, H, van Welsem, T, de Graaf, E L, Ontoso, D, Altelaar, A F M, San-Segundo, P A, Heck, A J R & van Leeuwen, F 2014, 'Flexibility in crosstalk between H2B ubiquitination and H3 methylation in vivo (Corrigendum)', EMBO Reports, vol. 15, no. 11, pp. 1220-1221. https://doi.org/10.15252/embr.201471110