The role of the transition between neutral and basic forms of human serum albumin in the kinetics of the binding to warfarin

Abstract

Between pH 6 and 9 in the kinetics of the binding of warfarin to human serum albumin a two-step mechanism operates: a diffusion-controlled step, followed by a much slower step during which the stable warfarin-albumin complex is formed. The association rate constant for the formation of the warfarin-albumin complex depends on the transition between neutral and basic forms of the albumin.