Characterization by 1H NMR spectroscopy of enzymically derived oligosaccharides from alkali-extractable wheat-flour arabinoxylan
Files
Publication date
1992
Authors
Vliegenthart, J.F.G.
Gruppen, H.
Hoffmann, R.A.
Kormelink, F.J.M.
Voragen, A.G.J.
Kamerling, J.P.
Editors
Advisors
Supervisors
DOI
Document Type
Article
Metadata
Show full item recordCollections
License
Abstract
Oligosaccharides obtained from alkali-extractable wheat-flour arabinoxylans by digestion with endo-(1-> 4)-beta-D-xylanase from Aspergillus awamori were fractionated by size-exclusion chromatography on Bio-Gel P-2 followed by high-performance anion-exchange chromatography, and subjected to monosaccharide analysis and 1H NMR spectroscopy. The results revealed (1 -> 4)-linked beta-D-xylopyrano-oligosaccharides partly 3- and/or 2,3-substituted with single alpha-L-arabinofuranosyl groups. The structures of 12 such arabinoxylan oligosaccharides were established.