Preparation and isolation of siRNA-loaded extracellular vesicles
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Publication date
2017
Authors
Vader, Pieter
Mäger, Imre
Lee, Yi
Nordin, Joel Z.
Andaloussi, Samir E L
Wood, Matthew J A
Editors
Advisors
Supervisors
Document Type
Part of book
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taverne
Abstract
RNA interference (RNAi) has tremendous potential for specific silencing of disease-causing genes. Its clinical usage however critically depends on the development of carrier systems that can transport the RNAimediating small interfering RNA (siRNA) molecules to the cytosol of target cells. Recent reports have suggested that extracellular vesicles (EVs) form a natural transport system through which biomolecules, including RNA, is exchanged between cells. Therefore, EVs are increasingly being considered as potential therapeutic siRNA delivery systems. In this chapter we describe a method for preparing siRNA-loaded EVs, including a robust, scalable method to isolate them from cell culture supernatants.
Keywords
Drug delivery, Exosomes, Extracellular vesicles, Microvesicles, siRNA, Size-exclusion chromatography, Taverne, Molecular Biology, Genetics, Journal Article
Citation
Vader, P, Mäger, I, Lee, Y, Nordin, J Z, Andaloussi, S E L & Wood, M J A 2017, Preparation and isolation of siRNA-loaded extracellular vesicles. in Exosomes and Microvesicles : Methods and Protocols. Methods in Molecular Biology, vol. 1545, Humana Press Inc., pp. 197-204. https://doi.org/10.1007/978-1-4939-6728-5_14