Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via Förster Resonance Energy Transfer
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Publication date
2010
Authors
Skajaa, Torjus
Zhao, Y.
Van Den Heuvel, Dave J.
Gerritsen, H.C.
Cormode, D.P.
Koole, R.
van Schooneveld, M.M.
Post, Jan Andries
Fisher, E.A.
Fayad, Z.A.
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Article
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Abstract
The study of lipoproteins, natural nanoparticles comprised of lipids and apolipoproteins that transport fats throughout the body, is of key importance to better understand, treat, and prevent cardiovascular disease. In the current study, we have developed a lipoprotein-based nanoparticle that consists of a quantum dot (QD) core and Cy5.5 labeled lipidic coating. The methodology allows judicious tuning of the QD/Cy5.5 ratio, which enabled us to optimize Frster resonance energy transfer (FRET) between the QD core and the Cy5.5-labeled coating. This phenomenon allowed us to study lipoprotein−lipoprotein interactions, lipid exchange dynamics, and the influence of apolipoproteins on these processes. Moreover, we were able to study HDL-cell interactions and exploit FRET to visualize HDL association with live macrophage cells.
Keywords
SDG 3 - Good Health and Well-being
Citation
Skajaa, T, Zhao, Y, van den Heuvel, D J, Gerritsen, H C, Cormode, D P, Koole, R, van Schooneveld, M M, Post, J A, Fisher, E A, Fayad, Z A, de Mello Donega, C, Meijerink, A & Mulder, W J M 2010, 'Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via Förster Resonance Energy Transfer', Nano Letters, vol. 10, pp. 5131-5138. https://doi.org/10.1021/nl1037903