Site-selective ser-hydrolase labelling with a luminescent organometallic NCN-platinum complex

Abstract

The synthesis, spectroscopic properties and protein-binding studies of a novel luminescent organometallic NCN-platinum complex are described. The luminescent organometallic complex was linked to a serine hydrolase reactive phosphonate group by means of click chemistry, and was ex-plotted in serine hydrolase specific-binding studies using gel electrophoresis. The NCN-platinum protein label was found to be a robust dye with absorbance and emission maxima between 374-380 and 482-493 nm, respectively, and quantum, yields between 0.04 and 0.15