Controlled aggregation of primary human pancreatic islet cells leads to glucose-responsive pseudoislets comparable to native islets
Publication date
2015-08
Authors
Hilderink, Janneke
Spijker, Siebe
Carlotti, Francoise
Lange, Lydia
Engelse, Marten
van Blitterswijk, Clemens
de Koning, Eelco
Karperien, Marcel
van Apeldoorn, Aart
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Article
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Abstract
Clinical islet transplantation is a promising treatment for patients with type 1 diabetes. However, pancreatic islets vary in size and shape affecting their survival and function after transplantation because of mass transport limitations. To reduce diffusion restrictions and improve islet cell survival, the generation of islets with optimal dimensions by dispersion followed by reassembly of islet cells, can help limit the length of diffusion pathways. This study describes a microwell platform that supports the controlled and reproducible production of three-dimensional pancreatic cell clusters of human donor islets. We observed that primary human islet cell aggregates with a diameter of 100-150m consisting of about 1000 cells best resembled intact pancreatic islets as they showed low apoptotic cell death (
Keywords
pseudoislets bioengineering, beta cells, islets, type 1 diabetes, HUMAN BETA-CELLS, MOLECULE N-CAM, INSULIN-SECRETION, MIN6 PSEUDOISLETS, TRANSPLANTATION, CULTURE, RELEASE, SEGREGATION, EXPRESSION, CLUSTERS, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
Citation
Hilderink, J, Spijker, S, Carlotti, F, Lange, L, Engelse, M, van Blitterswijk, C, de Koning, E, Karperien, M & van Apeldoorn, A 2015, 'Controlled aggregation of primary human pancreatic islet cells leads to glucose-responsive pseudoislets comparable to native islets', Journal of Cellular and Molecular Medicine, vol. 19, no. 8, pp. 1836-1846. https://doi.org/10.1111/jcmm.12555