Recombination in Escherichia coli V. Genetic analysis of recombinants from crosses with recipients deficient in ATP-dependent exonuclease activity

Abstract

A genetic analysis of recombinants from crosses with recombination-deficient recipients, lacking the ATP-dependent exonuclease activity, demonstrated differences in the inheritance pattern of donor markers compared with a Rec+ recipient. In particular the donor markers proximal to the transfer origin were inherited with a decreased frequency in the recombination-deficient recombinants. A mathematical analysis of the crosses showed that the observed deviations are mainy restricted to a reduced probability of exchange events in the region near the origin of the donor fragment.