Studies on the yeast nucleus I. The isolation of nuclei

Abstract

1. 1. A method for isolating pure cell-nuclei from the yeast Saccharomyces carlsbergensis is described. The yield of nuclei ranged from 70 to 80% as judged by the recovery of deoxyribonucleic acid.

2. 2. The morphology of the nuclei was dependent upon the ionic conditions in the medium which was used for the isolation.

3. 3. The isolated nuclei were characterized by high ratio of ribonucleic acid to deoxyribonucleic acid (3.7:1) and of protein to deoxyribonucleic acid (3.5:1).

4. 4. It is suggested that the nuclei may contain a substantial amount of nucleolar material, not consolidated into a single nucleolus, but more or less dispersed throughout the nucleus.