Some remarks regarding the manometric determination of pyridoxal phosphate

Abstract

In the manometric determination of pyridoxal phosphate (Gunsalus And Smith) continuous careful protection of the solutions from light is required. Stock solutions can be kept for at least one month if stored in the dark.

Pyridoxal phosphate added to alkaline extracts of myocardial tissue of rats could not be recovered completely. Therefore an internal standard was used. In this way we found in hearts of normal healthy rats an average of 4.6 μg pyridoxal phosphate per g wet tissue.