Lgr5+ve Stem/Progenitor Cells Contribute to Nephron Formation during Kidney Development
Publication date
2012-09-27
Authors
Barker, Nick
Rookmaaker, Maarten B
Kujala, Pekka
Ng, Annie
Leushacke, Marc
Snippert, Hugo
van de Wetering, Marc
Tan, Shawna
van Es, Johan H.
Huch, Meritxell
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Advisors
Supervisors
Document Type
Article
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cc_by_nc_nd
Abstract
Multipotent stem cells and their lineage-restricted progeny drive nephron formation within the developing kidney. Here, we document expression of the adult stem cell marker Lgr5 in the developing kidney and assess the stem/progenitor identity of Lgr5+ve cells via in vivo lineage tracing. The appearance and localization of Lgr5+ve cells coincided with that of the S-shaped body around embryonic day 14. Lgr5 expression remained restricted to cell clusters within developing nephrons in the cortex until postnatal day 7, when expression was permanently silenced. In vivo lineage tracing identified Lgr5 as a marker of a stem/progenitor population within nascent nephrons dedicated to generating the thick ascending limb of Henle@s loop and distal convoluted tubule. The Lgr5 surface marker and experimental models described here will be invaluable for deciphering the contribution of early nephron stem cells to developmental defects and for isolating human nephron progenitors as a prerequisite to evaluating their therapeutic potential
Keywords
General Biochemistry,Genetics and Molecular Biology
Citation
Barker, N, Rookmaaker, M B, Kujala, P, Ng, A, Leushacke, M, Snippert, H, van de Wetering, M, Tan, S, Van Es, J H, Huch, M, Poulsom, R, Verhaar, M C, Peters, P J & Clevers, H 2012, 'Lgr5 +ve Stem/Progenitor Cells Contribute to Nephron Formation during Kidney Development', Cell Reports [E], vol. 2, no. 3, pp. 540-552. https://doi.org/10.1016/j.celrep.2012.08.018