1H- and 13C-N.M.R. assignments for structural elements of xylose-containing N-linked oligosaccharides, using 1D- and 2D-N.M.R. experiments

Abstract

1H-N.m.r. and 13C-n.m.r. spectral assignments for synthetic β-D-Xyl-(1→2)-β-D-Man-OMe, β-D-Xyl-(1→2)-[α-D-Man-(1→3)]-β-D-Man-OMe, β-D-Xyl-(1→2)-[α-D-Man-(1→6)]-β-D-Man-OMe, and β-D-Xyl-(1→2)-[α-D-Man-(1→3)]-[α-D-Man-(1→6)]-β-D-Man-OMe, which are structural elements of xylose-containing carbohydrate chains from N-glycoproteins, have been made on the basis of 1D and 2D (DQF 1H-1H COSY, HOHAHA) 500-MHz 1H-n.m.r. spectroscopy and 1D 50-MHz 13C-n.m.r. spectroscopy, respectively.