The function of GORASPs in Golgi apparatus organization in vivo
Publication date
2020-09-07
Authors
Grond, Rianne
Veenendaal, Tineke
Duran, Juan M.
Raote, Ishier
van Es, Johan H
Corstjens, Sebastiaan
Delfgou, Laura
El Haddouti, Benaissa
Malhotra, Vivek
Rabouille, C
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Article
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Abstract
In vitro experiments have shown that GRASP65 (GORASP1) and GRASP55 (GORASP2) proteins function in stacking Golgi cisternae. However, in vivo depletion of GORASPs in metazoans has given equivocal results. We have generated a mouse lacking both GORASPs and find that Golgi cisternae remained stacked. However, the stacks are disconnected laterally from each other, and the cisternal cross-sectional diameters are significantly reduced compared with their normal counterparts. These data support earlier findings on the role of GORASPs in linking stacks, and we suggest that unlinking of stacks likely affects dynamic control of COPI budding and vesicle fusion at the rims. The net result is that cisternal cores remain stacked, but cisternal diameter is reduced by rim consumption.
Keywords
Cell Biology
Citation
Grond, R, Veenendaal, T, Duran, J M, Raote, I, van Es, J H, Corstjens, S, Delfgou, L, El Haddouti, B, Malhotra, V & Rabouille, C 2020, 'The function of GORASPs in Golgi apparatus organization in vivo', The Journal of cell biology, vol. 219, no. 9, e202004191. https://doi.org/10.1083/jcb.202004191