Degradation and habitat-dependent colonization of plastics in Caribbean coastal waters and sediments by bacterial communities

Abstract

This study investigates microbial colonization of plastics in Caribbean coastal waters. We deployed five polymer types, on set with a mild UV-pretreatment and one set without UV-pretreatment, for 4.5 months in the water column and sediment at two locations, and analyzed the epiplastic biofilms with 16S rRNA gene sequencing. While a significant influence of location and habitat was apparent, we could not detect notable effects related to polymer type or UV-pretreatment on microbial community composition. Nevertheless, potential plastic and hydrocarbon degraders constituted up to 43 % of sequences from epiplastic biofilms, suggesting an affinity for plastic. Indeed, utilizing 13C-labeled PE and PP, we determined incorporation of plastic-derived carbon into microbial biomass. We measured isotopically labeled fatty acids in incubations with 13C labeled plastics in both water column and sediments, whether virgin or pre-weathered with UV light. The apparent biodegradation of plastic in benthic habitats challenges the perception of marine sediments as a final sink for polyolefins.