Simultaneous Viral Whole-Genome Sequencing and Differential Expression Profiling in Respiratory Syncytial Virus Infection of Infants
Files
Publication date
2021-01-01
Authors
Lin, Gu-Lung
Golubchik, Tanya
Drysdale, Simon
O'Connor, Daniel
Jefferies, Kimberley
Brown, Anthony
de Cesare, Mariateresa
Bonsall, David
Ansari, M Azim
Aerssens, Jeroen
Editors
Advisors
Supervisors
Document Type
Article
Metadata
Show full item recordCollections
License
taverne
Abstract
Targeted metagenomics using strand-specific libraries with target enrichment is a sensitive, generalized approach to pathogen sequencing and transcriptome profiling. Using this method, we recovered 13 (76%) complete human respiratory syncytial virus (RSV) genomes from 17 clinical respiratory samples, reconstructed the phylogeny of the infecting viruses, and detected differential gene expression between 2 RSV subgroups, specifically, a lower expression of the P gene and a higher expression of the M2 gene in RSV-A than in RSV-B. This methodology can help to relate viral genetics to clinical phenotype and facilitate ongoing population-level RSV surveillance and vaccine development. Clinical Trials Registration. NCT03627572 and NCT03756766.
Keywords
RNA sequencing, differential gene expression, respiratory syncytial virus, transcriptome profiling, whole-genome sequencing, Infectious Diseases, Immunology and Allergy, Journal Article
Citation
Lin, G-L, Golubchik, T, Drysdale, S, O'Connor, D, Jefferies, K, Brown, A, de Cesare, M, Bonsall, D, Ansari, M A, Aerssens, J, Bont, L, Openshaw, P, Martinón-Torres, F, Bowden, R & Pollard, A J 2021, 'Simultaneous Viral Whole-Genome Sequencing and Differential Expression Profiling in Respiratory Syncytial Virus Infection of Infants', The Journal of infectious diseases, vol. 222, no. Supplement_7, pp. S666-S671. https://doi.org/10.1093/infdis/jiaa448