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Oxazolone-Induced Intestinal Inflammation in Adult Zebrafish

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Publication date

2017

Authors

Brugman, Sylvia
Nieuwenhuis, Edward E.S.ISNI 0000000393345368

Editors

Clausen, Björn E.
Laman, Jon D.

Advisors

Supervisors

DOI

https://doi.org/10.1007/978-1-4939-6786-5_21

Document Type

Part of book

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Abstract

Zebrafish are an excellent model for the study of intestinal immunity. The availability of several transgenic reporter fish for different innate and adaptive immune cells and the high homology in terms of gut function and morphology enables in depth analysis of the process of intestinal inflammation. Here, we describe a method to induce intestinal inflammation by intra-rectal injection of the hapten oxazolone in adult zebrafish.

Keywords

Crohn’s disease, Granulocytes, Gut barrier function, IBD, Intestinal inflammation, Intra-rectal injection, Oxazolone, Ulcerative colitis, Zebrafish, Taverne, Genetics, Molecular Biology

Citation

Brugman, S & Nieuwenhuis, EES 2017, Oxazolone-Induced Intestinal Inflammation in Adult Zebrafish. in B E Clausen & J D Laman (eds), Inflammation : Methods and Protocols. vol. III, Methods in Molecular Biology, vol. 1559, Springer, pp. 311-318. https://doi.org/10.1007/978-1-4939-6786-5_21

URI

https://dspace.library.uu.nl/handle/1874/348186