Androgen receptor signalling in macrophages promotes TREM-1-mediated prostate cancer cell line migration and invasion
Publication date
2020-12-01
Authors
Cioni, Bianca
Zaalberg, Anniek
van Beijnum, Judy R.
Melis, Monique H.M.
van Burgsteden, Johan
Muraro, Mauro J.
Hooijberg, Erik
Peters, Dennis
Hofland, Ingrid
Lubeck, Yoni
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Document Type
Article
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Abstract
The androgen receptor (AR) is the master regulator of prostate cancer (PCa) development, and inhibition of AR signalling is the most effective PCa treatment. AR is expressed in PCa cells and also in the PCa-associated stroma, including infiltrating macrophages. Macrophages have a decisive function in PCa initiation and progression, but the role of AR in macrophages remains largely unexplored. Here, we show that AR signalling in the macrophage-like THP-1 cell line supports PCa cell line migration and invasion in culture via increased Triggering Receptor Expressed on Myeloid cells-1 (TREM-1) signalling and expression of its downstream cytokines. Moreover, AR signalling in THP-1 and monocyte-derived macrophages upregulates IL-10 and markers of tissue residency. In conclusion, our data suggest that AR signalling in macrophages may support PCa invasiveness, and blocking this process may constitute one mechanism of anti-androgen therapy.
Keywords
Aged, Androgen Antagonists/pharmacology, Anilides/pharmacology, Biopsy, Blood Buffy Coat/cytology, Case-Control Studies, Cell Line, Tumor, Cell Movement/drug effects, Chemotherapy, Adjuvant, Coculture Techniques, Disease-Free Survival, Humans, Macrophages/immunology, Male, Middle Aged, Neoadjuvant Therapy, Neoplasm Invasiveness/immunology, Nitriles/pharmacology, Progression-Free Survival, Prostate/pathology, Prostatectomy, Prostatic Neoplasms/immunology, Receptors, Androgen/metabolism, Robotic Surgical Procedures, Signal Transduction/immunology, Single-Cell Analysis, THP-1 Cells, Tosyl Compounds/pharmacology, Triggering Receptor Expressed on Myeloid Cells-1/metabolism, Journal Article, Research Support, Non-U.S. Gov't
Citation
Cioni, B, Zaalberg, A, van Beijnum, J R, Melis, M H M, van Burgsteden, J, Muraro, M J, Hooijberg, E, Peters, D, Hofland, I, Lubeck, Y, de Jong, J, Sanders, J, Vivié, J, van der Poel, H G, de Boer, J P, Griffioen, A W, Zwart, W & Bergman, A M 2020, 'Androgen receptor signalling in macrophages promotes TREM-1-mediated prostate cancer cell line migration and invasion', Nature Communications, vol. 11, no. 1, 4498. https://doi.org/10.1038/s41467-020-18313-y