Oleic acid enhances proliferation and calcium mobilization of CD3/CD28 activated CD4+ T cells through incorporation into membrane lipids
Publication date
2024-09
Authors
von Hegedus, Johannes Hendrick
de Jong, Anja J.
Hoekstra, Anna T.
Spronsen, Eric
Zhu, Wahwah
Cabukusta, Birol
Kwekkeboom, Joanneke C.
Heijink, Marieke
Bos, Erik
Berkers, Celia R
Editors
Advisors
Supervisors
Document Type
Article
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cc_by
Abstract
Unsaturated fatty acids (UFA) are crucial for T-cell effector functions, as they can affect the growth, differentiation, survival, and function of T cells. Nonetheless, the mechanisms by which UFA affects T-cell behavior are ill-defined. Therefore, we analyzed the processing of oleic acid, a prominent UFA abundantly present in blood, adipocytes, and the fat pads surrounding lymph nodes, in CD4+ T cells. We found that exogenous oleic acid increases proliferation and enhances the calcium flux response upon CD3/CD28 activation. By using a variety of techniques, we found that the incorporation of oleic acid into membrane lipids, rather than regulation of cellular metabolism or TCR expression, is essential for its effects on CD4+ T cells. These results provide novel insights into the mechanism through which exogenous oleic acid enhances CD4+ T-cell function.
Keywords
CD4 T cells, Cellular proliferation, Fatty acids, Immune regulation, Metabolomics, Oleic acid, TCR, Immunology and Allergy, Immunology
Citation
von Hegedus, J H, de Jong, A J, Hoekstra, A T, Spronsen, E, Zhu, W, Cabukusta, B, Kwekkeboom, J C, Heijink, M, Bos, E, Berkers, C R, Giera, M A, Toes, R E M & Ioan-Facsinay, A 2024, 'Oleic acid enhances proliferation and calcium mobilization of CD3/CD28 activated CD4 + T cells through incorporation into membrane lipids', European Journal of Immunology, vol. 54, no. 9, 2350685. https://doi.org/10.1002/eji.202350685