A post-insertion strategy for surface functionalization of bacterial and mammalian cell-derived extracellular vesicles
Publication date
2021-04
Authors
Jiang, Linglei
Luirink, Joen
Kooijmans, Sander A A
van Kessel, Kok P M
Jong, Wouter
van Essen, Max
Seinen, Cor W
de Maat, Steven
de Jong, Olivier G.
Gitz-François, Jerney F F
Editors
Advisors
Supervisors
Document Type
Article
Metadata
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License
cc_by
Abstract
Extracellular vesicles (EVs) are nanoparticles which are released by cells from all three domains of life: Archaea, Bacteria and Eukarya. They can mediate cell-cell communication by transferring cargoes such as proteins and nucleic acids between cells. EVs receive great interest in both academia and industry as they have the potential to be natural drug carriers or vaccine candidates. However, limitations to their clinical translation exist as efficient isolation, loading, labelling and surface-engineering methods are lacking. In this article, we investigate a 'post-insertion' approach, which is commonly used in the functionalization of liposomes in the pharmaceutical field, on two different EV types: mammalian cell-derived EVs and bacteria-derived EVs. We aimed to find an easy and flexible approach to functionalize EVs, thereby improving the labelling, isolation, and surface-engineering.
Keywords
Extracellular vesicles, Outer membrane vesicles, Poly(ethylene glycol)lipids, Post-insertion, Biophysics, Biochemistry, Molecular Biology, SDG 3 - Good Health and Well-being
Citation
Jiang, L, Luirink, J, Kooijmans, S A A, van Kessel, K P M, Jong, W, van Essen, M, Seinen, C W, de Maat, S, de Jong, O G, Gitz-François, J F F, Hennink, W E, Vader, P & Schiffelers, R M 2021, 'A post-insertion strategy for surface functionalization of bacterial and mammalian cell-derived extracellular vesicles', Biochimica et Biophysica Acta - General Subjects, vol. 1865, no. 4, 129763, pp. 1-9. https://doi.org/10.1016/j.bbagen.2020.129763