Structural analysis of the α--D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme
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Publication date
2008
Authors
Van Leeuwen, S. M.
Kralj, S.
van Geel-Schutten, I.H.
Gerwig, G.J.
Dijkhuizen, L.
Kamerling, J.P.
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Abstract
The neutral exopolysaccharide EPS180 produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1→3, 1→6)-α-d-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and 1D/2D 1H and 13C NMR spectroscopy of the intact EPS180, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of EPS180, a composite model, that includes all identified structural features, was formulated as follows:
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van Leeuwen, S S, Kralj, S, van Geel-Schutten, I H, Gerwig, G J, Dijkhuizen, L & Kamerling, J P 2008, 'Structural analysis of the α--D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme', Carbohydrate Research, vol. 343, no. 7, pp. 1237-1250.