Combining two Meishan F2 crosses improves the detection of QTL on pig chromosomes 2, 4 and 6

Abstract

Genet Sel Evol. 2010 Nov 25;42(1):42. [Epub ahead of print] Combining two Meishan F2 crosses improves the detection of QTL on pig chromosomes 2, 4 and 6. Tortereau F, Gilbert H, Heuven HC, Bidanel JP, Groenen MA, Riquet J. Abstract ABSTRACT: BACKGROUND: In pig, a number of experiments have been set up to identify QTL and a multitude of chromosomal regions harbouring genes influencing traits of interest have been identified. However, the mapping resolution remains limited in most cases and the detected QTL are rather inaccurately located. Mapping accuracy can be improved by increasing the number of phenotyped and genotyped individuals and/or the number of informative markers. An alternative approach to overcome the limited power of individual studies is to combine data from two or more independent designs. METHODS: In the present study we report a combined analysis of two independent design (a French and a Dutch F2 experimental designs), with 2000 F2 individuals. The purpose was to further map QTL for growth and fatness on pig chromosomes 2, 4 and 6. Using QTLMap software, uni- and multiple-QTL detection analyses were applied separately on the two pedigrees and then on the combination of the two pedigrees. RESULTS: Joint analysis of the combined pedigree provides (1) greater significance of shared QTL, (2) exclusion of false suggestive QTL and (3) greater mapping precision for shared QTL. CONCLUSIONS: Combining two Meishan x European breeds F2 pedigrees improved the mapping of QTL compared to analysing pedigrees separately. Our work was facilitated by the access to raw phenotypic data and DNA of animals from both pedigrees and the combination of the two designs with the addition of new markers allowed us to fine map QTL without phenotyping additional animals. PMID: 21108822 [PubMed - as supplied by publisher]