PID-1 is a novel factor that operates during 21U-RNA biogenesis in Caenorhabditis elegans
Publication date
2014-04-01
Authors
de Albuquerque, Bruno F M
Simonis, M.J.
Cordeiro Rodrigues, Ricardo J
van Bergeijk, Petra
Waaijers, Selma
Kaaij, Lucas J T
Klein, Holger
Boxem, Mike
Ketting, René F
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Document Type
Article
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Abstract
The Piwi-piRNA pathway represents a small RNA-based mechanism responsible for the recognition and silencing of invading DNA. Biogenesis of piRNAs (21U-RNAs) is poorly understood. In Caenorhabditis elegans, the piRNA-binding Argonaute protein PRG-1 is the only known player acting downstream from precursor transcription. From a screen aimed at the isolation of piRNA-induced silencing-defective (Pid) mutations, we identified, among known Piwi pathway components, PID-1 as a novel player. PID-1 is a mostly cytoplasmic, germline-specific factor essential for 21U-RNA biogenesis, affecting an early step in the processing or transport of 21U precursor transcripts. We also show that maternal 21U-RNAs are essential to initiate silencing.
Keywords
Animals, Argonaute Proteins, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Methylation, Mutation, Protein Precursors, RNA Interference, RNA, Small Interfering, Transgenes
Citation
de Albuquerque, B F M, Luteijn, M J, Cordeiro Rodrigues, R J, van Bergeijk, P, Waaijers, S, Kaaij, L J T, Klein, H, Boxem, M & Ketting, R F 2014, 'PID-1 is a novel factor that operates during 21U-RNA biogenesis in Caenorhabditis elegans', Genes and Development, vol. 28, no. 7, pp. 683-688. https://doi.org/10.1101/gad.238220.114