Presence of a chlorophyll d-like pigment in Chlorella extracts

Abstract

Three chlorophyll a isomers (a₁, a₂ and a₃) were separated by the chromatography of Chlorella extracts on paper 1. One of these, chlorophyll (a₃) showed additional absorption bands at 688 and 455 mμ in diethyl ether. Chromatographic analysis could not decide whether these bands were due to a₃ or to a minor compound mixed with it. We investigated the question by exciting the fluorescence of a₃ at different wavelengths.