Protocol for culturing healthy primary endothelial cells isolated from research-grade human corneoscleral donor tissue
Publication date
2026-06-19
Authors
van der Krogt, Jeffrey M.A.
Dallinga, Marchien G.
van den Bogaerdt, Antoon J.
van der Meulen, Ivanka J.E.
van Buul, Jaap D.
Editors
Advisors
Supervisors
Document Type
Article
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cc_by
Abstract
Studying the function of healthy corneal endothelial cells has been challenging due to a variety of culturing strategies. Here, we present a standardized protocol for culturing primary human corneal endothelial cells, isolated from research-grade corneoscleral donor tissue, without the addition of mitotic enhancers. We describe the required enzymes, medium composition, cell density, and incubation times. Additionally, we use immunofluorescence microscopy to illustrate optimal outcomes and discuss troubleshooting procedures.
Keywords
General Neuroscience, General Immunology and Microbiology, General Biochemistry,Genetics and Molecular Biology
Citation
van der Krogt, J M A, Dallinga, M G, van den Bogaerdt, A J, van der Meulen, I J E & van Buul, J D 2026, 'Protocol for culturing healthy primary endothelial cells isolated from research-grade human corneoscleral donor tissue', STAR protocols, vol. 7, no. 2, 104535. https://doi.org/10.1016/j.xpro.2026.104535