Columbus Surpassed: Biophysical Aspects of How Stingless Bees Place an Egg Upright on Their Liquid Food

Abstract

The highly eusocial stingless bees (reviewed in [1, 2]) constitute a phylogenetically old group [3] within the Apidae. Probably related to this ancient origin is their mass provisioning of brood cells: they deposit all the food on which the larva will develop into the brood cell, prior to oviposition and subsequent closure of the cell. The other social members of the Apidae, the honeybees and the bumblebees, regularly visit the larvae in their cells to administer small amounts of liquid food at a time. This system is called progressive provisioning. Mass provisioning is practiced by almost all the other bees. However, while most of them have a solid type of larval food, the stingless bees produce a liquid food. In relation to this, their cylindrical brood cells are constructed vertically and are often arranged in horizontal combs. The food, regurgitated from the stomach, consists of a mixture of pollen, nectar, and glandular secretions [1, 2, 4]. After the workers have put this liquid food into the cell, the queen oviposits. Her egg, with its elongated shape, stands upright on the fluid.