Best practice standards for circular RNA research
Publication date
2022-10
Authors
Nielsen, Anne F.
Bindereif, Albrecht
Bozzoni, Irene
Hanan, Mor
Hansen, Thomas B.
Irimia, Manuel
Kadener, Sebastian
Kristensen, Lasse S.
Legnini, Ivano
Morlando, Mariangela
Editors
Advisors
Supervisors
Document Type
Article
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taverne
Abstract
Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors’ experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.
Keywords
RNA Splicing, RNA, Circular, RNA/genetics, Taverne, Molecular Biology, Biochemistry, Biotechnology, Cell Biology, Review, Journal Article
Citation
Nielsen, A F, Bindereif, A, Bozzoni, I, Hanan, M, Hansen, T B, Irimia, M, Kadener, S, Kristensen, L S, Legnini, I, Morlando, M, Jarlstad Olesen, M T, Pasterkamp, R J, Preibisch, S, Rajewsky, N, Suenkel, C & Kjems, J 2022, 'Best practice standards for circular RNA research', Nature Methods, vol. 19, no. 10, pp. 1208-1220. https://doi.org/10.1038/s41592-022-01487-2