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Asthmatic farm children show increased CD3+CD8low T-cells compared to non-asthmatic farm children

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Publication date

2017-10

Authors

Twardziok, Monika
Schröder, Paul C
Krusche, Johanna
Casaca, Vera I
Illi, Sabina
Böck, Andreas
Loss, Georg J
Kabesch, Michael
Toncheva, Antoaneta A
Roduit, Caroline

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DOI

https://doi.org/10.1016/j.clim.2017.09.009

Document Type

Article

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Utrecht University Repository
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taverne

Abstract

Several studies report an important role of CD8+ cytotoxic T-cells in atopy. Farm children show protection against atopy development, partly explained by CD4+ T-cell subtypes. Additional effects of CD8+ T-cells are unknown being investigated in this study within the PASTURE/EFRAIM birth cohort in PBMCs from farming and non-farming 6-year-old (N=76) German children. CD3+CD8+CD25+ T-cells were analyzed by flow cytometry. Genotyping of 17q21 locus-SNPs associated with childhood asthma was performed. No differences in CD8+ T-cell subsets were seen between farmers and non-farmers regardless of asthma. Among farmchildren, asthmatics displayed increased CD3+CD8low(CD25+) T-cells compared to non-asthmatics. Asthmatic farm children exhibited a lower PI-induced stimulatory capacity of CD3+CD8low(CD25+) cells and a lower IFN-γ secretion than non-asthmatic farm children. Among farm children with GSDMB and ORMDL3 risk alleles, asthmatics displayed higher CD3+CD8low cells than non-asthmatics. Our data indicates a specific role of CD8low T-cells in asthmatic farm children.

Keywords

CD8=+, CD8low, Childhood, farm-exposure, Taverne

Citation

Twardziok, M, Schröder, P C, Krusche, J, Casaca, V I, Illi, S, Böck, A, Loss, G J, Kabesch, M, Toncheva, A A, Roduit, C, Depner, M, Genuneit, J, Renz, H, Roponen, M, Weber, J, Braun-Fahrländer, C, Riedler, J, Lauener, R P, Vuitton, D A, Dalphin, J-C, Pekkanen, J, von Mutius, E, Schaub, B, Hyvärinen, A, Karvonen, A M, Kirjavainen, P V, Remes, S, Kaulek, V, Dalphin, M-L, Ege, M J, Pfefferle, P I, Doekes, G & PASTURE Study Group: 2017, 'Asthmatic farm children show increased CD3+CD8low T-cells compared to non-asthmatic farm children', Clinical Immunology, vol. 183, pp. 285-292. https://doi.org/10.1016/j.clim.2017.09.009

URI

http://hdl.handle.net/1874/360536