Reductive methylation labeling, from quantitative to structural proteomics
Publication date
2019-09
Authors
Liu, Z.
Zhou, Ye
Liu, Jing
Chen, Jin
Heck, Albert J.R.
Wang, Fangjun
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Advisors
Supervisors
Document Type
Article
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Abstract
Reductive methylation is a highly reactive and selective chemical labeling method and plays an important role in proteomic analysis. Various applications of reductive methylation have been developed and have substantially promoted the scope of proteomic analysis to date. Aside from a well-known method for introducing isotopes, this reaction could also be conducted under biological conditions and has little structural perturbations. In this paper, we reviewed the recent developments and applications of reductive methylation in quantitative proteomics and protein N-terminal profiling, along with the emerging applications in structural proteomics for monitoring protein structural/conformational changes under various conditions.
Keywords
Lysine reactivity profiling, Mass spectrometry, N-termini profiling, Quantitative proteomics, Reductive methylation, Stable isotope dimethyl labeling, Structural proteomics, Taverne, Analytical Chemistry, Spectroscopy
Citation
Liu, Z, Zhou, Y, Liu, J, Chen, J, Heck, A J R & Wang, F 2019, 'Reductive methylation labeling, from quantitative to structural proteomics', TrAC - Trends in Analytical Chemistry, vol. 118, pp. 771-778. https://doi.org/10.1016/j.trac.2019.07.009