Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue
Publication date
2022
Authors
Pei, Jia Yi
van den Dungen, Noortje A. M.
Asselbergs, Folkert W
Mokry, Michal
Harakalova, Magdalena
Editors
Horsfield, Julia
Marsman, Judith
Advisors
Supervisors
Document Type
Part of book
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License
taverne
Abstract
Chromatin immunoprecipitation and sequencing (ChIP-seq) is a well-established method to study the epigenetic profile at the genome-wide scale, including histone modifications and DNA-protein interactions. It provides valuable insights to better understand disease mechanisms. Here we present an optimized ChIP-seq protocol suitable for human cardiac tissues, especially the frozen biobanked small biopsy samples.
Keywords
Antibody, Cardiac tissues, Chromatin immunoprecipitation, Enhancers, Promoters, Sequencing, Small biopsy, Taverne, Molecular Biology, Genetics
Citation
Pei, J, van den Dungen, N A M, Asselbergs, F W, Mokry, M & Harakalova, M 2022, Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue. in J Horsfield & J Marsman (eds), Chromatin : Methods and Protocols. vol. 2458, Methods in Molecular Biology, vol. 2458, Humana Press, pp. 97-111. https://doi.org/10.1007/978-1-0716-2140-0_6