Detection of the Helicobacter pylori dupA gene is strongly affected by the PCR design
Publication date
2014-11
Authors
Abadi, Amin Talebi Bezmin
Loffeld, Ruud J L F
Constancia, Ashandra C
Wagenaar, Jaap
Kusters, Johannes G
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Document Type
Article
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Abstract
The Helicobacter pylori virulence gene dupA is usually detected by PCR, but the primer binding sites used are highly variable. Our newly designed qPCR against a conserved region of dupA was positive in 64.2% of 394 clinical isolates while the positivity rate of the commonly used PCRs ranged from 29.9% to 37.8%.
Keywords
Helicobacter pylori, dupA, Primer sets, Coronacrisis-Taverne
Citation
Abadi, A T B, Loffeld, R J L F, Constancia, A C, Wagenaar, J A & Kusters, J G 2014, 'Detection of the Helicobacter pylori dupA gene is strongly affected by the PCR design', Journal of Microbiological Methods, vol. 106, pp. 55-56. https://doi.org/10.1016/j.mimet.2014.07.027