Rhizobacteria-mediated induced systemic resistance (ISR) in Arabidopsis requires sensitivity to jasmonate and ethylene but is not accompanied by an increase in their production

Abstract

Plants develop an enhanced defensive capacity against a broad spectrum of plant pathogens after colonization of the roots by selected strains of nonpathogenic biocontrol bacteria. In Arabidopsis thaliana, this induced systemic resistance (ISR) functions independently of salicylic acid but requires an intact response to the plant hormones jasmonic acid (JA) and ethylene. To further investigate the roles of JA and ethylene in the ISR signalling pathway, the levels of these signalling molecules were determined in A. thaliana upon induction of ISR by Pseudomonas fluorescens WCS417r and subsequent challenge inoculation with Pseudomonas syringae pv. tomato DC3000. Upon treatment of the roots with ISR-inducing WCS417r bacteria, neither the JA content, nor the level of ethylene evolution was altered in systemically resistant leaves. Infiltration of leaves with WCS417r triggered the JA- and ethylene-dependent ISR pathway, but did not cause local changes in the production of either of these signalling molecules. These results indicate that rhizobacteria-mediated ISR is not based on the induction of changes in the biosynthesis of either JA or ethylene. However, in ISR-expressing plants the capacity to convert 1-aminocyclopropane-1-carboxylate (ACC) to ethylene was significantly enhanced, providing a greater potential to produce ethylene upon pathogen attack.