Detection of bacterial DNA in bile of cats with lymphocytic cholangitis
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Publication date
2012
Authors
Otte, C.
Gutiérrez, O.P.
Favier, R.P.
Rothuizen, J.
Penning, L.C.
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Document Type
Article
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Abstract
AbstractIn this study, we have successfully used molecular methods based on the amplification of the 16S ribosomal RNA gene on feline bile samples to show that bile of cats with LC is not sterile. This is probably due to the fact that the inflammatory process in the biliary tree causes dilatations. As a result, bacteria can easily migrate from the intestines via the common bile duct. The diversity of species identified and the presence of Helicobacter spp. DNA in both patients and controls suggests that bacteriobilia is secondary to the disease and is not the cause of LC.
Keywords
Cats, Hepatobiliary disease, Helicobacter spp., DGGE, 16s rDNA
Citation
Otte, C, Gutiérrez, O P, Favier, R P, Rothuizen, J & Penning, L C 2012, 'Detection of bacterial DNA in bile of cats with lymphocytic cholangitis', Veterinary Microbiology, vol. 156, no. 1-2, pp. 217-221. https://doi.org/10.1016/j.vetmic.2011.10.023