A procedure for parallel isolation of white blood cells, granulocyte and purified neutrophil suspensions from the peripheral blood of cattle
Publication date
1992-02-15
Authors
Kremer, W.D.J.
Noordhuizen-Stassen, E. N.
Henricks, Paul
de Man-van der Vliet, H.
Editors
Advisors
Supervisors
Document Type
Article
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taverne
Abstract
A rapid method is described for parallel isolation of white blood cells, granulocytes and purified neutrophils from peripheral blood of normal cattle. The mean recovery (±S.D.) of white blood cells, granulocytes and purified neutrophils isolated from peripheral blood of 13 cows was 66.4±12.6%, 68.7±20.0% and 38.0±20.9%, respectively. The mean purity of the isolated granulocyte and neutrophil suspensions was 94.0±3.8% and 95.0±6.0%, respectively. Viability of isolated cells was more than 97%.
Keywords
Taverne, Immunology, General Veterinary
Citation
Kremer, W D J, Noordhuizen-Stassen, E N, Henricks, P A J & van der Vliet, H 1992, 'A procedure for parallel isolation of white blood cells, granulocyte and purified neutrophil suspensions from the peripheral blood of cattle', Veterinary Immunology and Immunopathology, vol. 31, no. 1-2, pp. 189-193. https://doi.org/10.1016/0165-2427(92)90096-9