A 'catch-and-release' receptor for the cholera toxin
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Publication date
2019-10-01
Authors
Mahon, Clare S.
Wildsmith, Gemma C.
Haksar, Diksha
de Poel, Eyleen
Beekman, JM
Pieters, Roland J.
Webb, Michael E.
Turnbull, W. Bruce
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Article
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taverne
Abstract
Stimuli-responsive receptors for the recognition unit of the cholera toxin (CTB) have been prepared by attaching multiple copies of its natural carbohydrate ligand, the GM1 oligosaccharide, to a thermoresponsive polymer scaffold. Below their lower critical solution temperature (LCST), polymers complex CTB with nanomolar affinity. When heated above their LCST, polymers undergo a reversible coil to globule transition which renders a proportion of the carbohydrate recognition motifs inaccessible to CTB. This thermally-modulated decrease in the avidity of the material for the protein has been used to reversibly capture CTB from solution, enabling its convenient isolation from a complex mixture.
Keywords
Taverne, Physical and Theoretical Chemistry
Citation
Mahon, C S, Wildsmith, G C, Haksar, D, De Poel, E, Beekman, J M, Pieters, R J, Webb, M E & Turnbull, W B 2019, 'A 'catch-and-release' receptor for the cholera toxin', Faraday discussions, vol. 219, pp. 112-127. https://doi.org/10.1039/c9fd00017h