Cloning of a third nitrate reductase gene from the cyanobacterium Anacystis nidulans R2 using a shuttle cosmid library
Publication date
1984
Authors
Kuhlemeier, C.J.
Teeuwsen, V.J.P.
Janssen, M.J.T.
Arkel, G.A. van
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Document Type
Article
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Abstract
A strategy for gene cloning in the cyanobacterium Anacystis nidulans R2 was developed which made use of a gene library constructed in a shuttle cosmid vector. The method involved phenotypic complementation of mutants with pooled cosmid DNA. The development of the procedure and its application to the cloning of a third gene involved m nitrate reduction are described
Keywords
Photosynthesis, blue-green algae, mutants, complementation, recombinant DNA, phage λ vector, plasmid