Retinyl Ester Analysis by Orbitrap Mass Spectrometry
Publication date
2023-05-29
Authors
Jansen, Jeroen W A
Haaker, Maya W
Zaal, Esther A
Helms, J Bernd
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Document Type
Part of book
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taverne
Abstract
Retinoids are light-sensitive molecules that are normally detected by UV absorption techniques. Here we describe the identification and quantification of retinyl ester species by high-resolution mass spectrometry. Retinyl esters are extracted by the method of Bligh and Dyer and subsequently separated by HPLC in runs of 40 min. The retinyl esters are identified and quantified by mass spectrometry analysis. This procedure enables the highly sensitive detection and characterization of retinyl esters in biological samples such as hepatic stellate cells.
Keywords
Retinyl Esters/analysis, Vitamin A, Retinoids/analysis, Mass Spectrometry/methods, Chromatography, High Pressure Liquid/methods, Taverne
Citation
Jansen, J W A, Haaker, M W, Zaal, E A & Helms, J B 2023, Retinyl Ester Analysis by Orbitrap Mass Spectrometry. in Hepatic Stellate Cells : Methods and Protocols. Methods in Molecular Biology, Humana Press, pp. 67-77. https://doi.org/10.1007/978-1-0716-3207-9_5