Protoplasting of Aspergillus niger using Trichoderma harzianum culture medium after growth on cell walls of the target fungus
Publication date
2025-11
Authors
Moran Torres, Juan Pablo
Wösten, Han A B
Lugones, Luis G
Editors
Advisors
Supervisors
Document Type
Article
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Abstract
Protoplast yield from Aspergillus niger can be low when using commercial lytic enzymes from Trichoderma harzianum. This study shows that the culture medium of T. harzianum after growth on A. niger cell walls is much more effective in protoplasting than commercial lysing enzymes.
Keywords
Aspergillus niger, Cell wall degrading enzymes, Fungus, Protoplasting, Trichoderma harzianum, Microbiology, Molecular Biology, Microbiology (medical)
Citation
Torres, J P M, Wösten, H A B & Lugones, L G 2025, 'Protoplasting of Aspergillus niger using Trichoderma harzianum culture medium after growth on cell walls of the target fungus', Journal of Microbiological Methods, vol. 238, 107252. https://doi.org/10.1016/j.mimet.2025.107252