Immunocytochemistry on acid hydrolases in preputial gland cells

Abstract

Present knowledge of the in situ localization of acid hydrolases is mainly based on enzyme-cytochemical observations on acid phosphatase and aryl sulfatase. In rat preputial gland cells, β-glucuronidase and acid phosphatase were demon- strated this way in secretory granule-like lysosomes. we have applied immunocytochemistry, which in principle allows the detection of proteins at intracellular sites where they are not (yet) enzymatically active.