Prevalence of phylogroups and O25/ST131 in susceptible and extended-spectrum β-lactamase-producing Escherichia coli isolates, the Netherlands
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Publication date
2015-06
Authors
Overdevest, I T M A
Bergmans, A M C
Verweij, J J
Vissers, J
Bax, N
Snelders, E
Kluytmans, Jan A J W
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Abstract
To assess the distribution of phylogroups and O25/ST131 in the Netherlands, we performed a real-time polymerase chain reaction (PCR) on a collection of 108 wild-type Escherichia coli (WT-EC) and 134 extended-spectrum β-lactamase-producing E. coli (ESBL-EC). Phylogroup B2 was predominant, but ESBL-EC were less likely to belong to this phylogroup (48.5%) than were WT-EC (66.7%; p = 0.005). In WT-EC, phylogroups B2 and D seem to be more virulent, having a higher prevalence among midstream urine isolates and blood culture isolates, than in catheter-related urine isolates (83.3% and 87.9% vs. 61.9%; p 0.048). O25/ST131 is associated with ESBL production, being almost absent among phylogroup B2 WT-EC (61.5% vs. 5.6%; p < 0.001).
Keywords
Adult, Aged, Aged, 80 and over, Escherichia coli, Escherichia coli Infections, Female, Genotype, Humans, Male, Middle Aged, Netherlands, Prevalence, Prospective Studies, Real-Time Polymerase Chain Reaction, Retrospective Studies, Serogroup, beta-Lactamases, Journal Article
Citation
Overdevest, I T M A, Bergmans, A M C, Verweij, J J, Vissers, J, Bax, N, Snelders, E & Kluytmans, J A J W 2015, 'Prevalence of phylogroups and O25/ST131 in susceptible and extended-spectrum β-lactamase-producing Escherichia coli isolates, the Netherlands', Clinical Microbiology and Infection, vol. 21, no. 6, pp. 570.e1–570.e4. https://doi.org/10.1016/j.cmi.2015.02.020