Live imaging of cell division in 3D stem-cell organoid cultures
Publication date
2018-01-01
Authors
Bolhaqueiro, Ana C.F.
van Jaarsveld, Richard H.
Ponsioen, Bas
Overmeer, Rene M.
Snippert, HJG
Kops, Geert J P L
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Supervisors
Document Type
Part of book
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taverne
Abstract
Examining cell behavior in its correct tissue context is a major challenge in cell biology. The recent development of mammalian stem cell-based organoid cultures offers exciting opportunities to visualize dynamic cellular events in a 3D tissue-like setting. We describe here an approach for live imaging of cell division processes in intestinal organoid cultures derived from human and mouse adult stem cells. These approaches can be extended to the analysis of cellular events in diseased tissue, such as patient-derived tumor organoids.
Keywords
Aneuploidy, Chromosome, Mitosis, Organoid, Stem cell, Tumor, Taverne, Cell Biology, Journal Article, Research Support, Non-U.S. Gov't
Citation
Bolhaqueiro, A C F, van Jaarsveld, R H, Ponsioen, B, Overmeer, R M, Snippert, H J & Kops, G J P L 2018, Live imaging of cell division in 3D stem-cell organoid cultures. in Methods in Cell Biology. vol. 145, Methods in Cell Biology, vol. 145, Academic Press Inc., pp. 91-106. https://doi.org/10.1016/bs.mcb.2018.03.016