Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle
Publication date
2014-09-25
Authors
Kaloyanova, Dora
Vogels, M.W.
van Balkom, Bas W.M.
Helms, J Bernd
Editors
Schatten, Heide
Eisenstark, Abraham
Advisors
Supervisors
Document Type
Part of book
Metadata
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License
taverne
Abstract
Quantitative proteomics, based on stable isotope labeling by amino acids in cell culture (SILAC), can be used to identify host proteins involved in the intracellular interplay with pathogens. This method allows identification of proteins subject to degradation or upregulation in response to intracellular infection. It can also be used to study intracellular dynamics (trafficking) of proteins in response to the infection. Here, we describe the analysis of changes in protein profiles determined in Golgi-enriched fractions isolated from cells that were either mock-infected or infected with Salmonella typhimurium. Using the SILAC approach we were able to identify 105 proteins in Golgi-enriched fractions that were significantly changed in their abundance as a result of Salmonella infection.
Keywords
Host–pathogen interactions, Salmonella typhimurium, SILAC, Cellular fractionation, Golgi membrane enrichment, Taverne
Citation
Kaloyanova, D, Vogels, M, van Balkom, B W M & Helms, J B 2014, Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle. in H Schatten & A Eisenstark (eds), Salmonella : Methods and Protocols. 2 edn, Methods in Molecular Biology, vol. 1225, Humana Press, New York, pp. 29-45. https://doi.org/10.1007/978-1-4939-1625-2_2