Identification of serotonin 5-HT3 recognition sites by radioligand binding in NG108-15 neuroblastoma-glioma cells

Abstract

The 5-HT (5-hydroxytryptamine, serotonin) receptors have been subdivided into 3 main classes termed 5-HT₁, 5-HT₂ and 5-HT₃. Although well characterized in various isolated tissue models, 5-HT₃ receptors, in contrast to 5-HT₁ and 5-HT₂ receptors, have not yet been identified by means of radioligand binding techniques. We now report on the identification of 5-HT₃ recognition sites in NG 108-15 neuroblastomaglioma cells done with [³H]ICS 205-930, a potent and selective 5-HT₃ receptor antagonist.