From imprinting efforts to new concepts in controlled release of proteins

Abstract

The aim of this thesis was to develop a unique, innovative and very challenging concept to synthesize polymer nanoparticles displaying the imprints of membrane proteins (PINAPLES) on their surface. Despite several efforts to achieve the right conditions for protein imprinting, the proof of concept could not be unambiguously demonstrated. However, the synthesis of the protein-macromonomers led to a promising and highly attractive method for transient immobilization of proteins in hydrogels and subsequent triggered extra- and intracellular release. The next logical step is to down scale the system to obtain nanoparticles that can act as a carrier system (with targeting and prolonged circulation properties) and release the immobilized protein intracellularly upon reduction by glutathione.