Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming
Publication date
2019-11-19
Authors
Hoogendijk, Arie J
Pourfarzad, Farzin
Aarts, Cathelijn E M
Tool, Anton T J
Hiemstra, Ida H
Grassi, Luigi
Frontini, Mattia
Meijer, Alexander B
van den Biggelaar, Maartje
Kuijpers, Taco W
Editors
Advisors
Supervisors
Document Type
Article
Metadata
Show full item recordCollections
License
cc_by_nc_nd
Abstract
Human neutrophilic granulocytes form the largest pool of innate immune cells for host defense against bacterial and fungal pathogens. The dynamic changes that accompany the metamorphosis from a proliferating myeloid progenitor cell in the bone marrow into a mature non-dividing polymorphonuclear blood cell have remained poorly defined. Using mass spectrometry-based quantitative proteomics combined with transcriptomic data, we report on the dynamic changes of five developmental stages in the bone marrow and blood. Integration of transcriptomes and proteome unveils highly dynamic and differential interactions between RNA and protein kinetics during human neutrophil development, which can be linked to functional maturation of typical end-stage blood neutrophil killing activities.
Keywords
neutrophil development, granule proteins, proteomics, transcriptomics, granulopoiesis
Citation
Hoogendijk, A J, Pourfarzad, F, Aarts, C E M, Tool, A T J, Hiemstra, I H, Grassi, L, Frontini, M, Meijer, A B, van den Biggelaar, M & Kuijpers, T W 2019, 'Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming', Cell Reports, vol. 29, no. 8, pp. 2505-2519.e4. https://doi.org/10.1016/j.celrep.2019.10.082